Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
15710 | 5' | -56.9 | NC_004065.1 | + | 118511 | 0.71 | 0.729379 |
Target: 5'- gCCUGUCGCUCGGGuucGGCaucuucguUCGAGGa -3' miRNA: 3'- aGGGUAGCGAGCCUc--CCGau------AGCUCC- -5' |
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15710 | 5' | -56.9 | NC_004065.1 | + | 147186 | 0.72 | 0.71038 |
Target: 5'- cCCCGUCGCgaUUGGAGacGCUG-CGAGGa -3' miRNA: 3'- aGGGUAGCG--AGCCUCc-CGAUaGCUCC- -5' |
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15710 | 5' | -56.9 | NC_004065.1 | + | 170468 | 0.73 | 0.612973 |
Target: 5'- gCCCGUCGCcaucggcuUCGGGGGGUcuUCGGuGGa -3' miRNA: 3'- aGGGUAGCG--------AGCCUCCCGauAGCU-CC- -5' |
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15710 | 5' | -56.9 | NC_004065.1 | + | 163341 | 0.74 | 0.554685 |
Target: 5'- cUCCUGUCuCUUgGGGGGGCUGUgGGGGg -3' miRNA: 3'- -AGGGUAGcGAG-CCUCCCGAUAgCUCC- -5' |
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15710 | 5' | -56.9 | NC_004065.1 | + | 198990 | 0.75 | 0.545109 |
Target: 5'- cUCCUggCGCUCGGcAGGGCcgcggcCGAGGg -3' miRNA: 3'- -AGGGuaGCGAGCC-UCCCGaua---GCUCC- -5' |
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15710 | 5' | -56.9 | NC_004065.1 | + | 179031 | 1.11 | 0.002996 |
Target: 5'- cUCCCAUCGCUCGGAGGGCUAUCGAGGg -3' miRNA: 3'- -AGGGUAGCGAGCCUCCCGAUAGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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