Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 15710 | 5' | -56.9 | NC_004065.1 | + | 31291 | 0.66 | 0.931725 |
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Target: 5'- aCCCGUaCGacaCGcGAGGGUUG-CGAGGa -3' miRNA: 3'- aGGGUA-GCga-GC-CUCCCGAUaGCUCC- -5' |
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| 15710 | 5' | -56.9 | NC_004065.1 | + | 81808 | 0.66 | 0.954938 |
|
Target: 5'- aUCUCGUa-CUCGGAGGGgaAgguguagauuugacgUCGGGGa -3' miRNA: 3'- -AGGGUAgcGAGCCUCCCgaU---------------AGCUCC- -5' |
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| 15710 | 5' | -56.9 | NC_004065.1 | + | 20813 | 0.66 | 0.953432 |
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Target: 5'- uUCCaaCAUCGUccuUCucGAGGGUcGUCGAGGa -3' miRNA: 3'- -AGG--GUAGCG---AGc-CUCCCGaUAGCUCC- -5' |
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| 15710 | 5' | -56.9 | NC_004065.1 | + | 80561 | 0.66 | 0.936032 |
|
Target: 5'- aCCCggagggcuucgggGUCGauagcCUCGGGGGGgaGUcCGAGGa -3' miRNA: 3'- aGGG-------------UAGC-----GAGCCUCCCgaUA-GCUCC- -5' |
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| 15710 | 5' | -56.9 | NC_004065.1 | + | 109825 | 0.66 | 0.929753 |
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Target: 5'- cCUCGUCGCcguccugcugcggCGGGGGGCcAUCGcgcAGGu -3' miRNA: 3'- aGGGUAGCGa------------GCCUCCCGaUAGC---UCC- -5' |
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| 15710 | 5' | -56.9 | NC_004065.1 | + | 140730 | 0.66 | 0.953432 |
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Target: 5'- -gCCG-CGCUCGGucaacguGGGCaagGUgGAGGa -3' miRNA: 3'- agGGUaGCGAGCCu------CCCGa--UAgCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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