Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15718 | 3' | -58.7 | NC_004065.1 | + | 159940 | 0.7 | 0.671716 |
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Target: 5'- -gCUCGUCGCC-UCGGGG---UCCGCg -3' miRNA: 3'- ugGAGUAGCGGuAGCCCCaccAGGUG- -5' |
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| 15718 | 3' | -58.7 | NC_004065.1 | + | 59175 | 0.71 | 0.632574 |
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Target: 5'- -gUUCAUCGUCAUCGGGGgacguugaaGGcCCGCc -3' miRNA: 3'- ugGAGUAGCGGUAGCCCCa--------CCaGGUG- -5' |
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| 15718 | 3' | -58.7 | NC_004065.1 | + | 170467 | 0.72 | 0.583686 |
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Target: 5'- uGCC-CGUCGCCAucggcuUCGGGG-GGUCUuCg -3' miRNA: 3'- -UGGaGUAGCGGU------AGCCCCaCCAGGuG- -5' |
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| 15718 | 3' | -58.7 | NC_004065.1 | + | 109824 | 0.72 | 0.56431 |
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Target: 5'- cCCUCGUCGCCGUccugcugcggCGGGG-GG-CCAUc -3' miRNA: 3'- uGGAGUAGCGGUA----------GCCCCaCCaGGUG- -5' |
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| 15718 | 3' | -58.7 | NC_004065.1 | + | 109879 | 0.73 | 0.516724 |
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Target: 5'- cACCggCGUCGCCgcggccgcGUCGGGG-GGUCC-Cg -3' miRNA: 3'- -UGGa-GUAGCGG--------UAGCCCCaCCAGGuG- -5' |
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| 15718 | 3' | -58.7 | NC_004065.1 | + | 173954 | 1.09 | 0.002421 |
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Target: 5'- aACCUCAUCGCCAUCGGGGUGGUCCACg -3' miRNA: 3'- -UGGAGUAGCGGUAGCCCCACCAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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