Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15723 | 5' | -58.8 | NC_004065.1 | + | 162580 | 0.71 | 0.618089 |
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Target: 5'- cCUCGGcCGAcaGGUCCgUCGUGUGCGGg -3' miRNA: 3'- -GAGUC-GCUccUCGGGaAGCACGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 15520 | 0.71 | 0.608385 |
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Target: 5'- gCUCGGUGAGGAauGCCaCUUCGggacGCGUGc -3' miRNA: 3'- -GAGUCGCUCCU--CGG-GAAGCa---CGCGCc -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 83299 | 0.73 | 0.522471 |
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Target: 5'- gUgGGCGAcGGAGCUCUcgUCGUcgaGCGCGGc -3' miRNA: 3'- gAgUCGCU-CCUCGGGA--AGCA---CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 143061 | 0.74 | 0.485738 |
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Target: 5'- uCUgGGCGAGGGGCgCUUCGgggGCGaCGa -3' miRNA: 3'- -GAgUCGCUCCUCGgGAAGCa--CGC-GCc -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 138026 | 0.76 | 0.353261 |
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Target: 5'- -gCGGCGcacGGAGCCCUUCG-GgGCGGu -3' miRNA: 3'- gaGUCGCu--CCUCGGGAAGCaCgCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 171790 | 1.09 | 0.00266 |
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Target: 5'- cCUCAGCGAGGAGCCCUUCGUGCGCGGc -3' miRNA: 3'- -GAGUCGCUCCUCGGGAAGCACGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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