Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15723 | 5' | -58.8 | NC_004065.1 | + | 32658 | 0.67 | 0.837067 |
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Target: 5'- -gCAG-GAGGAGUUCcUCGcggGCGCGGu -3' miRNA: 3'- gaGUCgCUCCUCGGGaAGCa--CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 24969 | 0.67 | 0.858981 |
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Target: 5'- gUCGGuCGuGGgugggcgucccucGGCCUUUUGUGUGUGGg -3' miRNA: 3'- gAGUC-GCuCC-------------UCGGGAAGCACGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 24550 | 0.69 | 0.759914 |
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Target: 5'- -gCAGCGGagacGAGCCCcUCGUcgggaucGCGCGGu -3' miRNA: 3'- gaGUCGCUc---CUCGGGaAGCA-------CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 15520 | 0.71 | 0.608385 |
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Target: 5'- gCUCGGUGAGGAauGCCaCUUCGggacGCGUGc -3' miRNA: 3'- -GAGUCGCUCCU--CGG-GAAGCa---CGCGCc -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 6240 | 0.71 | 0.637519 |
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Target: 5'- -cCGGCugGAGGuAGUCCUgUCGUGCgGCGGg -3' miRNA: 3'- gaGUCG--CUCC-UCGGGA-AGCACG-CGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 1852 | 0.66 | 0.880687 |
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Target: 5'- aUCAGCGAGc-GUCCUcuguUCGcccaGCGCGGu -3' miRNA: 3'- gAGUCGCUCcuCGGGA----AGCa---CGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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