Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15723 | 5' | -58.8 | NC_004065.1 | + | 6240 | 0.71 | 0.637519 |
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Target: 5'- -cCGGCugGAGGuAGUCCUgUCGUGCgGCGGg -3' miRNA: 3'- gaGUCG--CUCC-UCGGGA-AGCACG-CGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 115422 | 0.7 | 0.694533 |
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Target: 5'- -gUAGCGAGGgcgauaccAGCCC-UCGUagggcacuccgccGCGCGGg -3' miRNA: 3'- gaGUCGCUCC--------UCGGGaAGCA-------------CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 158370 | 0.7 | 0.69549 |
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Target: 5'- -gCuGCuGAGGAgGCCCUUCGUccCGCGGa -3' miRNA: 3'- gaGuCG-CUCCU-CGGGAAGCAc-GCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 107746 | 0.7 | 0.705028 |
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Target: 5'- cCUCGGcCGGcGGAuGCCCcaggUCGgugucgGCGCGGg -3' miRNA: 3'- -GAGUC-GCU-CCU-CGGGa---AGCa-----CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 57783 | 0.69 | 0.733279 |
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Target: 5'- cCUUA-CGAGGGGCCgUUCGUGa-CGGg -3' miRNA: 3'- -GAGUcGCUCCUCGGgAAGCACgcGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 171790 | 1.09 | 0.00266 |
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Target: 5'- cCUCAGCGAGGAGCCCUUCGUGCGCGGc -3' miRNA: 3'- -GAGUCGCUCCUCGGGAAGCACGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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