Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15723 | 5' | -58.8 | NC_004065.1 | + | 133982 | 0.66 | 0.887286 |
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Target: 5'- aUCGGCGGccGGGGCCCgugcccaCGcaCGCGGg -3' miRNA: 3'- gAGUCGCU--CCUCGGGaa-----GCacGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 138295 | 0.66 | 0.887286 |
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Target: 5'- -aCAGgGAGGccguggacgcGGCCCUgaucgCGUGCGaGGc -3' miRNA: 3'- gaGUCgCUCC----------UCGGGAa----GCACGCgCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 109843 | 0.66 | 0.880687 |
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Target: 5'- -gCGGCGGGGGGCCaucgCGcagGUGcCGGg -3' miRNA: 3'- gaGUCGCUCCUCGGgaa-GCa--CGC-GCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 66526 | 0.66 | 0.880687 |
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Target: 5'- gCUguGCGuGGAGCgCCUgCGcuucaaccGCGCGGc -3' miRNA: 3'- -GAguCGCuCCUCG-GGAaGCa-------CGCGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 125746 | 0.67 | 0.85971 |
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Target: 5'- cCUCAGacAGG-GCCCggcgUCGUGUaucGCGGg -3' miRNA: 3'- -GAGUCgcUCCuCGGGa---AGCACG---CGCC- -5' |
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| 15723 | 5' | -58.8 | NC_004065.1 | + | 113749 | 0.7 | 0.705028 |
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Target: 5'- -cCGGCGAGGAGgCCga-GggacaGCGCGGc -3' miRNA: 3'- gaGUCGCUCCUCgGGaagCa----CGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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