Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1574 | 5' | -53.2 | NC_001347.2 | + | 192036 | 0.73 | 0.82816 |
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Target: 5'- aCGAGCGGCagCGGCGacGACGguggCGGCGa -3' miRNA: 3'- -GCUCGUUG--GCUGCaaCUGCaa--GCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 179 | 0.73 | 0.836296 |
|
Target: 5'- gCGGGCGuguggcggguguGCCGGCGgggUGugGUggcggggugugUCGGCGg -3' miRNA: 3'- -GCUCGU------------UGGCUGCa--ACugCA-----------AGCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 1427 | 0.74 | 0.793995 |
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Target: 5'- --cGCAGCCGACcc-GACGUUgGGCGa -3' miRNA: 3'- gcuCGUUGGCUGcaaCUGCAAgCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 172695 | 0.76 | 0.690185 |
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Target: 5'- gGAGCcGCCGACGcUGGCGcugCGGCu -3' miRNA: 3'- gCUCGuUGGCUGCaACUGCaa-GCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 188818 | 1.08 | 0.010897 |
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Target: 5'- uCGAGCAACCGACGUUGACGUUCGGCGc -3' miRNA: 3'- -GCUCGUUGGCUGCAACUGCAAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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