Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1574 | 5' | -53.2 | NC_001347.2 | + | 140898 | 0.66 | 0.992876 |
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Target: 5'- aGAGCAGCCgcGACGaggUGGCG---GGCGc -3' miRNA: 3'- gCUCGUUGG--CUGCa--ACUGCaagCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 166406 | 0.66 | 0.994518 |
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Target: 5'- uGGGCcACCGuGCGgugacccUUGGCGUgUCGGCu -3' miRNA: 3'- gCUCGuUGGC-UGC-------AACUGCA-AGCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 96807 | 0.66 | 0.994595 |
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Target: 5'- aGAGCcaccagguGCCGGCGacUGACGccggcaUCGGCa -3' miRNA: 3'- gCUCGu-------UGGCUGCa-ACUGCa-----AGCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 171715 | 0.66 | 0.994595 |
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Target: 5'- gCGAGUugguGACCGAgGcgGugGUUUGGgGc -3' miRNA: 3'- -GCUCG----UUGGCUgCaaCugCAAGCCgC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 76443 | 0.66 | 0.994595 |
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Target: 5'- uGGGCgAACCGACGcagaUGAUGcagggcacgCGGCGu -3' miRNA: 3'- gCUCG-UUGGCUGCa---ACUGCaa-------GCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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