Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1574 | 5' | -53.2 | NC_001347.2 | + | 197547 | 0.67 | 0.985015 |
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Target: 5'- cCGcAGCGugGCCGGCG-UGGCGgccgaCGGCa -3' miRNA: 3'- -GC-UCGU--UGGCUGCaACUGCaa---GCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 203897 | 0.68 | 0.974453 |
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Target: 5'- aCGAGCAGCCGccGCGU--GCGgauaGGCa -3' miRNA: 3'- -GCUCGUUGGC--UGCAacUGCaag-CCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 208030 | 0.67 | 0.988148 |
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Target: 5'- uGGGCGaugggACCGACGUcggagucgcGGCGUUCguuGGCGc -3' miRNA: 3'- gCUCGU-----UGGCUGCAa--------CUGCAAG---CCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 209741 | 0.69 | 0.958227 |
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Target: 5'- gGAGCAccguggguGCCGGCGguaUGACGgccaguucuugcgcUUCGGCc -3' miRNA: 3'- gCUCGU--------UGGCUGCa--ACUGC--------------AAGCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 229658 | 0.73 | 0.836296 |
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Target: 5'- gCGGGCGuguggcggguguGCCGGCGgggUGugGUggcggggugugUCGGCGg -3' miRNA: 3'- -GCUCGU------------UGGCUGCa--ACugCA-----------AGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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