Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1574 | 5' | -53.2 | NC_001347.2 | + | 2364 | 0.68 | 0.968986 |
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Target: 5'- uGGGCGcGCUGGCGcuggGACGcgagUCGGCGc -3' miRNA: 3'- gCUCGU-UGGCUGCaa--CUGCa---AGCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 2006 | 0.68 | 0.981288 |
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Target: 5'- gCGAGCGGCacggaGACGgaGGCcgcCGGCGg -3' miRNA: 3'- -GCUCGUUGg----CUGCaaCUGcaaGCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 1961 | 0.71 | 0.912833 |
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Target: 5'- gGGGCcGgCGACGggGACGg-CGGCGg -3' miRNA: 3'- gCUCGuUgGCUGCaaCUGCaaGCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 1427 | 0.74 | 0.793995 |
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Target: 5'- --cGCAGCCGACcc-GACGUUgGGCGa -3' miRNA: 3'- gcuCGUUGGCUGcaaCUGCAAgCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 179 | 0.73 | 0.836296 |
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Target: 5'- gCGGGCGuguggcggguguGCCGGCGgggUGugGUggcggggugugUCGGCGg -3' miRNA: 3'- -GCUCGU------------UGGCUGCa--ACugCA-----------AGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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