Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1574 | 5' | -53.2 | NC_001347.2 | + | 197547 | 0.67 | 0.985015 |
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Target: 5'- cCGcAGCGugGCCGGCG-UGGCGgccgaCGGCa -3' miRNA: 3'- -GC-UCGU--UGGCUGCaACUGCaa---GCCGc -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 34305 | 0.67 | 0.986652 |
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Target: 5'- aCGGuGUcGCCacCGUUGACGUgggCGGCGa -3' miRNA: 3'- -GCU-CGuUGGcuGCAACUGCAa--GCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 174021 | 0.67 | 0.986652 |
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Target: 5'- gGAGaaaacGCCGGCGgaGACGa-CGGCGg -3' miRNA: 3'- gCUCgu---UGGCUGCaaCUGCaaGCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 112626 | 0.67 | 0.98786 |
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Target: 5'- aGAGUcgcGCCGACGguaauaggccggUGAUGguggCGGCGg -3' miRNA: 3'- gCUCGu--UGGCUGCa-----------ACUGCaa--GCCGC- -5' |
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| 1574 | 5' | -53.2 | NC_001347.2 | + | 76443 | 0.66 | 0.994595 |
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Target: 5'- uGGGCgAACCGACGcagaUGAUGcagggcacgCGGCGu -3' miRNA: 3'- gCUCG-UUGGCUGCa---ACUGCaa-------GCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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