Results 81 - 86 of 86 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
15746 | 3' | -60.2 | NC_004065.1 | + | 133503 | 0.66 | 0.833404 |
Target: 5'- cUCCUCggacucguCGUCGuccuCCGACGAGGAGGa -3' miRNA: 3'- uAGGAG--------GCGGCcgu-GGCUGCUCCUUC- -5' |
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15746 | 3' | -60.2 | NC_004065.1 | + | 138815 | 0.66 | 0.841191 |
Target: 5'- cUUCUCCGgCGGCGCCGAUGc----- -3' miRNA: 3'- uAGGAGGCgGCCGUGGCUGCuccuuc -5' |
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15746 | 3' | -60.2 | NC_004065.1 | + | 55442 | 0.66 | 0.841191 |
Target: 5'- gGUCUUCgaGUCGGCGCUG--GGGGAAGg -3' miRNA: 3'- -UAGGAGg-CGGCCGUGGCugCUCCUUC- -5' |
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15746 | 3' | -60.2 | NC_004065.1 | + | 113485 | 0.66 | 0.841191 |
Target: 5'- aAUCCUCCacgGUCGGCACCguacgucucuuuGACGccGAGGc -3' miRNA: 3'- -UAGGAGG---CGGCCGUGG------------CUGCucCUUC- -5' |
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15746 | 3' | -60.2 | NC_004065.1 | + | 143715 | 0.66 | 0.841191 |
Target: 5'- -aCCag-GUCGGCGCCGGCGAGcGAGa -3' miRNA: 3'- uaGGaggCGGCCGUGGCUGCUCcUUC- -5' |
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15746 | 3' | -60.2 | NC_004065.1 | + | 162161 | 0.66 | 0.841191 |
Target: 5'- gAUCggCCGCCGGCugCGGCGcAGc--- -3' miRNA: 3'- -UAGgaGGCGGCCGugGCUGC-UCcuuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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