Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15746 | 3' | -60.2 | NC_004065.1 | + | 197125 | 0.67 | 0.788687 |
|
Target: 5'- -gCCUCCGacccccgacaggaCGGCAUCucgGACGAGGAGc -3' miRNA: 3'- uaGGAGGCg------------GCCGUGG---CUGCUCCUUc -5' |
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| 15746 | 3' | -60.2 | NC_004065.1 | + | 197486 | 0.66 | 0.817344 |
|
Target: 5'- ----aCCGCCGGCG-CGACGGGGccGu -3' miRNA: 3'- uaggaGGCGGCCGUgGCUGCUCCuuC- -5' |
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| 15746 | 3' | -60.2 | NC_004065.1 | + | 200367 | 0.66 | 0.800681 |
|
Target: 5'- cGUCCUCUu----CGCCGGCGAGGAGGg -3' miRNA: 3'- -UAGGAGGcggccGUGGCUGCUCCUUC- -5' |
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| 15746 | 3' | -60.2 | NC_004065.1 | + | 201295 | 0.72 | 0.509536 |
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Target: 5'- -cCCUCCGgCGGC-CgGGgGAGGAGGa -3' miRNA: 3'- uaGGAGGCgGCCGuGgCUgCUCCUUC- -5' |
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| 15746 | 3' | -60.2 | NC_004065.1 | + | 210615 | 0.73 | 0.42151 |
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Target: 5'- -aCCUCCGCCGaCGCCGGCGGGu--- -3' miRNA: 3'- uaGGAGGCGGCcGUGGCUGCUCcuuc -5' |
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| 15746 | 3' | -60.2 | NC_004065.1 | + | 212759 | 0.67 | 0.774678 |
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Target: 5'- -aCCUCCGgCGGUGaaGAaGAGGAGGu -3' miRNA: 3'- uaGGAGGCgGCCGUggCUgCUCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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