Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15746 | 5' | -57.6 | NC_004065.1 | + | 116272 | 0.66 | 0.899199 |
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Target: 5'- cGACgaGCUCUUgugUCAGUCGGuuGGGGa- -3' miRNA: 3'- -CUG--CGAGAGa--AGUCAGCCccUCCCag -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 187951 | 0.67 | 0.892879 |
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Target: 5'- cGGCGCUCUgUaccgcguaCAG-CGGGaGAGGGUg -3' miRNA: 3'- -CUGCGAGAgAa-------GUCaGCCC-CUCCCAg -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 44913 | 0.67 | 0.879604 |
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Target: 5'- --aGCUCUg-UCAGU-GGGcGGGGGUCu -3' miRNA: 3'- cugCGAGAgaAGUCAgCCC-CUCCCAG- -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 115623 | 0.68 | 0.843689 |
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Target: 5'- --gGCUgUCUUCAGgCGGGGuaggcuggacggcauGGGUCu -3' miRNA: 3'- cugCGAgAGAAGUCaGCCCCu--------------CCCAG- -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 86367 | 0.68 | 0.826942 |
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Target: 5'- cGAgGCUCUCggcCAcGUCGGGGuaGGUCu -3' miRNA: 3'- -CUgCGAGAGaa-GU-CAGCCCCucCCAG- -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 176438 | 0.69 | 0.766244 |
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Target: 5'- cGCGUcuUCUCUgucgaUCuggguGUCGGGGAGGGa- -3' miRNA: 3'- cUGCG--AGAGA-----AGu----CAGCCCCUCCCag -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 115555 | 0.71 | 0.694286 |
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Target: 5'- aGCGUcugUCUCUgucugucuguuugucUCAGUCGGGGAaaucaGGGUUg -3' miRNA: 3'- cUGCG---AGAGA---------------AGUCAGCCCCU-----CCCAG- -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 138241 | 0.71 | 0.67081 |
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Target: 5'- aGGCGCUCgccgCcGUCGaGGAGGGUCg -3' miRNA: 3'- -CUGCGAGagaaGuCAGCcCCUCCCAG- -5' |
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| 15746 | 5' | -57.6 | NC_004065.1 | + | 156946 | 1.09 | 0.003094 |
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Target: 5'- cGACGCUCUCUUCAGUCGGGGAGGGUCu -3' miRNA: 3'- -CUGCGAGAGAAGUCAGCCCCUCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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