Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15752 | 3' | -57 | NC_004065.1 | + | 130882 | 0.71 | 0.656607 |
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Target: 5'- cGGGCGCCggcgUGAGGuCCUCAuGAGGuGUg -3' miRNA: 3'- -CUCGCGGa---GUUCCuGGAGUuCUCC-CA- -5' |
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| 15752 | 3' | -57 | NC_004065.1 | + | 92047 | 0.72 | 0.646614 |
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Target: 5'- uGGCGCgCUCGAGGcAUCUCAcgcgcgcucGGAGGGc -3' miRNA: 3'- cUCGCG-GAGUUCC-UGGAGU---------UCUCCCa -5' |
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| 15752 | 3' | -57 | NC_004065.1 | + | 204334 | 0.73 | 0.556071 |
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Target: 5'- aGAGCGCCgugguagUCGGGGACgUCGugauGGGGGGa -3' miRNA: 3'- -CUCGCGG-------AGUUCCUGgAGU----UCUCCCa -5' |
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| 15752 | 3' | -57 | NC_004065.1 | + | 143297 | 0.75 | 0.453222 |
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Target: 5'- aGGCgGCgUCGGGGGCCggggCAGGAGGGg -3' miRNA: 3'- cUCG-CGgAGUUCCUGGa---GUUCUCCCa -5' |
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| 15752 | 3' | -57 | NC_004065.1 | + | 80565 | 0.77 | 0.368669 |
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Target: 5'- gGAGgGCUUCGGGGucgauaGCCUCGGGGGGGa -3' miRNA: 3'- -CUCgCGGAGUUCC------UGGAGUUCUCCCa -5' |
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| 15752 | 3' | -57 | NC_004065.1 | + | 153943 | 1.07 | 0.004501 |
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Target: 5'- cGAGCGCCUCAAGGACCUCAAGAGGGUc -3' miRNA: 3'- -CUCGCGGAGUUCCUGGAGUUCUCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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