Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1576 | 5' | -55.2 | NC_001347.2 | + | 98334 | 0.66 | 0.950655 |
Target: 5'- aGGGaUCUUgugGGCuUCCAcGUGCCGAc-- -3' miRNA: 3'- -UCCgAGAAa--CCG-AGGU-CACGGCUacu -5' |
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1576 | 5' | -55.2 | NC_001347.2 | + | 139573 | 0.67 | 0.932449 |
Target: 5'- gAGGCgCgagGGCUCUAcGUGCCGGc-- -3' miRNA: 3'- -UCCGaGaaaCCGAGGU-CACGGCUacu -5' |
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1576 | 5' | -55.2 | NC_001347.2 | + | 199772 | 0.7 | 0.814907 |
Target: 5'- cGGUUUcUUGGCggcgCCGGUGCCGccGGu -3' miRNA: 3'- uCCGAGaAACCGa---GGUCACGGCuaCU- -5' |
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1576 | 5' | -55.2 | NC_001347.2 | + | 212029 | 0.72 | 0.722129 |
Target: 5'- cGGCaUCUUUGGCUCUAuGUGCCu---- -3' miRNA: 3'- uCCG-AGAAACCGAGGU-CACGGcuacu -5' |
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1576 | 5' | -55.2 | NC_001347.2 | + | 188367 | 1.09 | 0.004376 |
Target: 5'- gAGGCUCUUUGGCUCCAGUGCCGAUGAg -3' miRNA: 3'- -UCCGAGAAACCGAGGUCACGGCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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