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miRNA: 3'- -AGGAugGCUaGCUuCUC-GUCUCGUUG- -5'
miRNA: 3'- aGGAUGGcuaGCUUCUCGUCUCg-------UUG- -5'
miRNA: 3'- aGGA-------------UGGCUa-------GCuUCUCGUC-UCGUUG- -5'
miRNA: 3'- aGGAUGGCUAGCuucuCGuCUcGUUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 15760 | 3' | -51.6 | NC_004065.1 | + | 23988 | 0.66 | 0.995419 |
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Target: 5'- gUCCU--CGG-CGAuGAGaCAGAGCGACg -3' miRNA: 3'- -AGGAugGCUaGCUuCUC-GUCUCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 114902 | 0.66 | 0.994682 |
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Target: 5'- cCCUACC---CGGAGAGCGGGGauaucccaAGCg -3' miRNA: 3'- aGGAUGGcuaGCUUCUCGUCUCg-------UUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 144235 | 0.66 | 0.994603 |
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Target: 5'- aCCUcaccguacggaugACCGAgaacgccaCGcAGAGCAGuAGCAGCa -3' miRNA: 3'- aGGA-------------UGGCUa-------GCuUCUCGUC-UCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 113014 | 0.66 | 0.997146 |
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Target: 5'- cCCUGCCGAUCGu---GCcGAuCAACg -3' miRNA: 3'- aGGAUGGCUAGCuucuCGuCUcGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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