miRNA: 3'- agGAUGGCuaGCUUCUCguC-UCGUUG- -5'
miRNA: 3'- aGG--AUGGCUAGCUUCU-CGUcUCGUUG- -5'
miRNA: 3'- -AGGAUGGC--UAGCUU-CUCGUCuCGUUG- -5'
miRNA: 3'- -AGGAUGGCUAGCUUCUCGUCUCGUUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15760 | 3' | -51.6 | NC_004065.1 | + | 130846 | 0.72 | 0.901656 |
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Target: 5'- -aCUACCG--CGAGGAGguGcAGCAGCa -3' miRNA: 3'- agGAUGGCuaGCUUCUCguC-UCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 112780 | 0.72 | 0.901656 |
|
Target: 5'- cCCagUGCCGGUUGAAGAaGCGcAGCAGCc -3' miRNA: 3'- aGG--AUGGCUAGCUUCU-CGUcUCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 56595 | 0.75 | 0.762797 |
|
Target: 5'- cUUCUGCCGucGUCGAGcGAGCAG-GCGGCc -3' miRNA: 3'- -AGGAUGGC--UAGCUU-CUCGUCuCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 147956 | 1.11 | 0.008399 |
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Target: 5'- aUCCUACCGAUCGAAGAGCAGAGCAACu -3' miRNA: 3'- -AGGAUGGCUAGCUUCUCGUCUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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