Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15760 | 3' | -51.6 | NC_004065.1 | + | 223789 | 0.67 | 0.9906 |
Target: 5'- gCUUGCCGuAUCGcuuGAGCAGucuagcuAGCGGCu -3' miRNA: 3'- aGGAUGGC-UAGCuu-CUCGUC-------UCGUUG- -5' |
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15760 | 3' | -51.6 | NC_004065.1 | + | 224242 | 0.66 | 0.997329 |
Target: 5'- cUCCUGcgcCCGG-CGAgcgGGAggggaagcaacagcuGCGGAGCAGCg -3' miRNA: 3'- -AGGAU---GGCUaGCU---UCU---------------CGUCUCGUUG- -5' |
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15760 | 3' | -51.6 | NC_004065.1 | + | 227499 | 0.72 | 0.907942 |
Target: 5'- gUCUACCGAggCGAAGAGCGGuuUGAUg -3' miRNA: 3'- aGGAUGGCUa-GCUUCUCGUCucGUUG- -5' |
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15760 | 3' | -51.6 | NC_004065.1 | + | 229392 | 0.68 | 0.988021 |
Target: 5'- cCCaccCCGGggucCGAAGAGCGGugaAGCGACu -3' miRNA: 3'- aGGau-GGCUa---GCUUCUCGUC---UCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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