miRNA: 3'- -AGGaUGG-CUAGCUUCUCGucuCGUUG- -5'
miRNA: 3'- aGGAUGGCUa--GCUUCucGUCUC-GUUG- -5'
miRNA: 3'- aGGAUGGCU--AGCU--UCUCGUCUcGUUG- -5'
miRNA: 3'- -AGGAUGGCUAGCUUCUCGUCUCGUUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15760 | 3' | -51.6 | NC_004065.1 | + | 7653 | 0.7 | 0.961176 |
|
Target: 5'- gUUCaACCgGAUCGAAGAGCu--GCGACu -3' miRNA: 3'- -AGGaUGG-CUAGCUUCUCGucuCGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 31819 | 0.69 | 0.973614 |
|
Target: 5'- gCCUGCCGGacgCGGAGuuCAGGGcCGACc -3' miRNA: 3'- aGGAUGGCUa--GCUUCucGUCUC-GUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 62285 | 0.69 | 0.973614 |
|
Target: 5'- cCCUAUCGAccUUGAagAGAGCGGGuCGACa -3' miRNA: 3'- aGGAUGGCU--AGCU--UCUCGUCUcGUUG- -5' |
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| 15760 | 3' | -51.6 | NC_004065.1 | + | 147956 | 1.11 | 0.008399 |
|
Target: 5'- aUCCUACCGAUCGAAGAGCAGAGCAACu -3' miRNA: 3'- -AGGAUGGCUAGCUUCUCGUCUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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