Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15760 | 5' | -50.2 | NC_004065.1 | + | 23287 | 0.66 | 0.999365 |
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Target: 5'- cCCGUaGCCgcggCCUUCGuUAUCGGcGUg -3' miRNA: 3'- -GGCAaCGGaca-GGAAGCuAUAGCU-CA- -5' |
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| 15760 | 5' | -50.2 | NC_004065.1 | + | 151527 | 0.67 | 0.997652 |
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Target: 5'- gCCGcUGCCgg-CCgggCGGUGUCGGGc -3' miRNA: 3'- -GGCaACGGacaGGaa-GCUAUAGCUCa -5' |
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| 15760 | 5' | -50.2 | NC_004065.1 | + | 153411 | 0.68 | 0.996739 |
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Target: 5'- aCGUUGCC-GUCUaUCGAc-UCGAGg -3' miRNA: 3'- gGCAACGGaCAGGaAGCUauAGCUCa -5' |
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| 15760 | 5' | -50.2 | NC_004065.1 | + | 184836 | 0.7 | 0.986843 |
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Target: 5'- uCCGUUGCUggGUUCggcCGAgggAUCGAGUc -3' miRNA: 3'- -GGCAACGGa-CAGGaa-GCUa--UAGCUCA- -5' |
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| 15760 | 5' | -50.2 | NC_004065.1 | + | 218191 | 0.77 | 0.79417 |
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Target: 5'- uUCGUUGCCUGUgCUagcgguaacguuaUCGAUAUCGAu- -3' miRNA: 3'- -GGCAACGGACAgGA-------------AGCUAUAGCUca -5' |
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| 15760 | 5' | -50.2 | NC_004065.1 | + | 147993 | 1.11 | 0.012933 |
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Target: 5'- aCCGUUGCCUGUCCUUCGAUAUCGAGUg -3' miRNA: 3'- -GGCAACGGACAGGAAGCUAUAGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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