Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
15763 | 5' | -59.6 | NC_004065.1 | + | 43331 | 0.66 | 0.880061 |
Target: 5'- --gGcCCGGCGGGGAcgccccACG-CG-CCGCGu -3' miRNA: 3'- ccaC-GGCCGCCCCU------UGCaGUaGGCGU- -5' |
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15763 | 5' | -59.6 | NC_004065.1 | + | 120171 | 0.66 | 0.873296 |
Target: 5'- aGUGCCaccugGGUgaGGGGAGCGaCG-CCGCGc -3' miRNA: 3'- cCACGG-----CCG--CCCCUUGCaGUaGGCGU- -5' |
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15763 | 5' | -59.6 | NC_004065.1 | + | 76753 | 0.66 | 0.866339 |
Target: 5'- cGGcGCCGGCGGccu-CGUCAUCguccggCGCAu -3' miRNA: 3'- -CCaCGGCCGCCccuuGCAGUAG------GCGU- -5' |
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15763 | 5' | -59.6 | NC_004065.1 | + | 81997 | 0.66 | 0.866339 |
Target: 5'- --cGgCGGCGGGGAagGCGccCGUgCCGCu -3' miRNA: 3'- ccaCgGCCGCCCCU--UGCa-GUA-GGCGu -5' |
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15763 | 5' | -59.6 | NC_004065.1 | + | 221861 | 0.66 | 0.859195 |
Target: 5'- uGGUGCUGGUGGGaaucguucuucaGAugGcCuUCUGCGu -3' miRNA: 3'- -CCACGGCCGCCC------------CUugCaGuAGGCGU- -5' |
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15763 | 5' | -59.6 | NC_004065.1 | + | 387 | 0.66 | 0.878052 |
Target: 5'- --cGCCGGCcGGGAugGcgCAcgagacgguguuuuUCCGCGc -3' miRNA: 3'- ccaCGGCCGcCCCUugCa-GU--------------AGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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