Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15763 | 5' | -59.6 | NC_004065.1 | + | 203509 | 0.74 | 0.452621 |
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Target: 5'- uGUGCCGGCGGGGucCGUCG--CGUg -3' miRNA: 3'- cCACGGCCGCCCCuuGCAGUagGCGu -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 207490 | 0.66 | 0.873296 |
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Target: 5'- aGGUGggauCCGGaaCGGGGAAuacCGUUGUCCGgGu -3' miRNA: 3'- -CCAC----GGCC--GCCCCUU---GCAGUAGGCgU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 208021 | 0.69 | 0.715056 |
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Target: 5'- aGUGCCGGCGGcGAugacCGUCGgcguggCCGUg -3' miRNA: 3'- cCACGGCCGCCcCUu---GCAGUa-----GGCGu -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 221861 | 0.66 | 0.859195 |
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Target: 5'- uGGUGCUGGUGGGaaucguucuucaGAugGcCuUCUGCGu -3' miRNA: 3'- -CCACGGCCGCCC------------CUugCaGuAGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 229153 | 0.73 | 0.487898 |
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Target: 5'- -aUGCCGGCGGGGcgcccucgcccGGCaGUCAugcgcUCCGCGu -3' miRNA: 3'- ccACGGCCGCCCC-----------UUG-CAGU-----AGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 229512 | 0.69 | 0.686675 |
|
Target: 5'- --cGCUGGCGGGGcGCcUCAccccgccuUCCGCGg -3' miRNA: 3'- ccaCGGCCGCCCCuUGcAGU--------AGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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