Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15763 | 5' | -59.6 | NC_004065.1 | + | 35629 | 0.75 | 0.371019 |
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Target: 5'- cGGcGgCGGCGGGG-AUGUCAUCgGCGa -3' miRNA: 3'- -CCaCgGCCGCCCCuUGCAGUAGgCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 33013 | 0.7 | 0.638667 |
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Target: 5'- cGGUcGUCGGUc-GGAuCGUCAUCCGCAa -3' miRNA: 3'- -CCA-CGGCCGccCCUuGCAGUAGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 30587 | 0.66 | 0.851869 |
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Target: 5'- --cGCC-GCGGcGAGCGccUCGUCCGCGu -3' miRNA: 3'- ccaCGGcCGCCcCUUGC--AGUAGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 29970 | 0.68 | 0.78741 |
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Target: 5'- cGGUGCUGGCGGcGGcGGCGg---CgGCAg -3' miRNA: 3'- -CCACGGCCGCC-CC-UUGCaguaGgCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 19566 | 0.72 | 0.515184 |
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Target: 5'- gGGUGUCGGUGGcGGcgguAGCGUCggCUGCGa -3' miRNA: 3'- -CCACGGCCGCC-CC----UUGCAGuaGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 387 | 0.66 | 0.878052 |
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Target: 5'- --cGCCGGCcGGGAugGcgCAcgagacgguguuuuUCCGCGc -3' miRNA: 3'- ccaCGGCCGcCCCUugCa-GU--------------AGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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