Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15763 | 5' | -59.6 | NC_004065.1 | + | 127487 | 0.69 | 0.686675 |
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Target: 5'- cGG-GCgCGGC-GGGAGCGUUGUCCaGCu -3' miRNA: 3'- -CCaCG-GCCGcCCCUUGCAGUAGG-CGu -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 103703 | 0.69 | 0.702815 |
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Target: 5'- cGGUgGgCGGCGGGGAGCGccuggcgggcgugaUCGUgaGCAc -3' miRNA: 3'- -CCA-CgGCCGCCCCUUGC--------------AGUAggCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 119612 | 0.69 | 0.714118 |
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Target: 5'- --cGCCGGCGGcGGAGgagacgcCGUCggCCGUg -3' miRNA: 3'- ccaCGGCCGCC-CCUU-------GCAGuaGGCGu -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 208021 | 0.69 | 0.715056 |
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Target: 5'- aGUGCCGGCGGcGAugacCGUCGgcguggCCGUg -3' miRNA: 3'- cCACGGCCGCCcCUu---GCAGUa-----GGCGu -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 166151 | 0.68 | 0.769914 |
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Target: 5'- gGGUGaCGuGCGGGuGGugGUCcUUCGCGa -3' miRNA: 3'- -CCACgGC-CGCCC-CUugCAGuAGGCGU- -5' |
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| 15763 | 5' | -59.6 | NC_004065.1 | + | 147490 | 1.1 | 0.002204 |
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Target: 5'- uGGUGCCGGCGGGGAACGUCAUCCGCAu -3' miRNA: 3'- -CCACGGCCGCCCCUUGCAGUAGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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