Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15777 | 3' | -52.3 | NC_004065.1 | + | 162896 | 0.66 | 0.99464 |
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Target: 5'- gCGUCcaggcgCGCGCCGCUGUccagcacGGCg -3' miRNA: 3'- aGCAGua----GCGCGGCGACAacuau--CUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 88047 | 0.66 | 0.99464 |
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Target: 5'- gUCGUCAUCGcCGUCGCcuccgGAgggaGGACc -3' miRNA: 3'- -AGCAGUAGC-GCGGCGacaa-CUa---UCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 117067 | 0.66 | 0.995375 |
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Target: 5'- aCGUUcggcCGCGgCGCUGUcGGUGGAg -3' miRNA: 3'- aGCAGua--GCGCgGCGACAaCUAUCUg -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 189798 | 0.66 | 0.995375 |
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Target: 5'- gUCGUCGUCGUccaccCCGUUGUucUGuaccaaAUAGACg -3' miRNA: 3'- -AGCAGUAGCGc----GGCGACA--AC------UAUCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 166818 | 0.66 | 0.995375 |
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Target: 5'- cCGUCAUCGUGCaGCgGUUGGa---- -3' miRNA: 3'- aGCAGUAGCGCGgCGaCAACUaucug -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 20403 | 0.66 | 0.995443 |
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Target: 5'- cCGUCGgucuaaagcagacaGUGCCGCUGUUGAcGGuguACg -3' miRNA: 3'- aGCAGUag------------CGCGGCGACAACUaUC---UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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