Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15777 | 3' | -52.3 | NC_004065.1 | + | 62030 | 0.72 | 0.88315 |
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Target: 5'- gUCGcCAUCGCuGCUGCUGcUGGUGGuGCc -3' miRNA: 3'- -AGCaGUAGCG-CGGCGACaACUAUC-UG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 105916 | 0.72 | 0.868754 |
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Target: 5'- gUGUCgAUCGCGgCGCUGagccgGAUGGGCc -3' miRNA: 3'- aGCAG-UAGCGCgGCGACaa---CUAUCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 78611 | 0.74 | 0.812012 |
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Target: 5'- aCGUCGUCGCGCCGCcugagGUgaacgcGGUcGGCg -3' miRNA: 3'- aGCAGUAGCGCGGCGa----CAa-----CUAuCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 13093 | 0.76 | 0.677691 |
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Target: 5'- aUUGUCAUCGCGUcucuccugagCGCUGUUGAaucuacagAGACa -3' miRNA: 3'- -AGCAGUAGCGCG----------GCGACAACUa-------UCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 97192 | 0.77 | 0.647107 |
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Target: 5'- cUCGUCGUCGCGCUGCgccu-GUAGGCc -3' miRNA: 3'- -AGCAGUAGCGCGGCGacaacUAUCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 138373 | 1.1 | 0.008911 |
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Target: 5'- gUCGUCAUCGCGCCGCUGUUGAUAGACc -3' miRNA: 3'- -AGCAGUAGCGCGGCGACAACUAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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