Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15777 | 3' | -52.3 | NC_004065.1 | + | 196730 | 0.68 | 0.980918 |
|
Target: 5'- gCGaCGUCGCGcCCGCUGUUcuccuGGCa -3' miRNA: 3'- aGCaGUAGCGC-GGCGACAAcuau-CUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 197545 | 0.67 | 0.990704 |
|
Target: 5'- aUCGUCAagggacacuggCGCGCCGaCgaccUGGUGGGCa -3' miRNA: 3'- -AGCAGUa----------GCGCGGC-Gaca-ACUAUCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 198140 | 0.66 | 0.992887 |
|
Target: 5'- -gGUCAUCGcCGCCGCcaugGGUGG-Cg -3' miRNA: 3'- agCAGUAGC-GCGGCGacaaCUAUCuG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 202916 | 0.67 | 0.984785 |
|
Target: 5'- gUCGUCAUCGuCGUCGUUGgcguucggGGUccuggggagaGGACg -3' miRNA: 3'- -AGCAGUAGC-GCGGCGACaa------CUA----------UCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 206562 | 0.7 | 0.944933 |
|
Target: 5'- gUCGUCAUCGUcgcgaGCCGCgcgcauacgGAUAGAg -3' miRNA: 3'- -AGCAGUAGCG-----CGGCGacaa-----CUAUCUg -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 229623 | 0.7 | 0.936557 |
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Target: 5'- aUGUCucUCGCGCCGCg--UGGUAG-Ca -3' miRNA: 3'- aGCAGu-AGCGCGGCGacaACUAUCuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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