Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15777 | 3' | -52.3 | NC_004065.1 | + | 96988 | 0.67 | 0.990704 |
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Target: 5'- cCGUcCGUCGCGCUGCUGc-----GACg -3' miRNA: 3'- aGCA-GUAGCGCGGCGACaacuauCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 135580 | 0.67 | 0.984962 |
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Target: 5'- cCGUCAccuccuucgaacacaCGCGCCGCUGgg---AGACg -3' miRNA: 3'- aGCAGUa--------------GCGCGGCGACaacuaUCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 35001 | 0.68 | 0.982934 |
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Target: 5'- aCGUCGUCGC-CCGUcGUUGGc-GGCg -3' miRNA: 3'- aGCAGUAGCGcGGCGaCAACUauCUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 196730 | 0.68 | 0.980918 |
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Target: 5'- gCGaCGUCGCGcCCGCUGUUcuccuGGCa -3' miRNA: 3'- aGCaGUAGCGC-GGCGACAAcuau-CUG- -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 18706 | 0.68 | 0.976359 |
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Target: 5'- gUCGUCgAagGUGCCGCUGUcGAUcacAGAg -3' miRNA: 3'- -AGCAG-UagCGCGGCGACAaCUA---UCUg -5' |
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| 15777 | 3' | -52.3 | NC_004065.1 | + | 97192 | 0.77 | 0.647107 |
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Target: 5'- cUCGUCGUCGCGCUGCgccu-GUAGGCc -3' miRNA: 3'- -AGCAGUAGCGCGGCGacaacUAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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