Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15805 | 3' | -57.7 | NC_004065.1 | + | 149065 | 0.67 | 0.890617 |
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Target: 5'- -gCGGCAGGCCgaucGGGGUCguCGUcacgaUCGUc -3' miRNA: 3'- ggGCUGUUCGG----CCCCAGguGCA-----AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 7314 | 0.67 | 0.896276 |
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Target: 5'- uCCUGACGaucgAGCCGGuGGUaCCGgacgcggcggagcCGUUCGg -3' miRNA: 3'- -GGGCUGU----UCGGCC-CCA-GGU-------------GCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 16439 | 0.67 | 0.908826 |
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Target: 5'- gCCCGagcccacuaGCGAGCCc-GGUaCCACGUUCGc -3' miRNA: 3'- -GGGC---------UGUUCGGccCCA-GGUGCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 76306 | 0.67 | 0.914476 |
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Target: 5'- uCUCGuCGAGCCGGaGGaUCCGCcg-CGUg -3' miRNA: 3'- -GGGCuGUUCGGCC-CC-AGGUGcaaGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 112959 | 0.66 | 0.919912 |
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Target: 5'- aCCGACAGcGCCGcGG-CCgaACGUUCGc -3' miRNA: 3'- gGGCUGUU-CGGCcCCaGG--UGCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 59008 | 0.66 | 0.925135 |
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Target: 5'- -gCGAaAGGCCaGGGUCgGCgGUUCGUg -3' miRNA: 3'- ggGCUgUUCGGcCCCAGgUG-CAAGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 99970 | 0.66 | 0.934935 |
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Target: 5'- gCCgCGGCGugcucgGGCCGcGGGUCguCGUcuUCGUc -3' miRNA: 3'- -GG-GCUGU------UCGGC-CCCAGguGCA--AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 101188 | 0.66 | 0.934935 |
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Target: 5'- -aCGugAacagguugcGGCCGGGGUCgGCGgugaaacauUUCGUg -3' miRNA: 3'- ggGCugU---------UCGGCCCCAGgUGC---------AAGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 121461 | 0.66 | 0.943881 |
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Target: 5'- uUCGACGaccaggucGGUCGGcGcGUCCGCGggCGUg -3' miRNA: 3'- gGGCUGU--------UCGGCC-C-CAGGUGCaaGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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