Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15805 | 3' | -57.7 | NC_004065.1 | + | 138695 | 0.7 | 0.79214 |
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Target: 5'- uCCCGAUAAGCgGGcgaucguggagcGGUCCucAgGUUCGUg -3' miRNA: 3'- -GGGCUGUUCGgCC------------CCAGG--UgCAAGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 31685 | 0.7 | 0.774678 |
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Target: 5'- cCCCGGCGAGCUGaGGGggaucuggccguUCCGCGgcaUGUg -3' miRNA: 3'- -GGGCUGUUCGGC-CCC------------AGGUGCaa-GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 26145 | 0.7 | 0.765772 |
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Target: 5'- -aCGA-GAGCCGGGGUCUGCGUgccagcCGUc -3' miRNA: 3'- ggGCUgUUCGGCCCCAGGUGCAa-----GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 152327 | 0.71 | 0.738443 |
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Target: 5'- -gCGACAGGaacaCGGGGcCCACGcUCGg -3' miRNA: 3'- ggGCUGUUCg---GCCCCaGGUGCaAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 198450 | 0.72 | 0.652809 |
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Target: 5'- aCCGACGucGCCGc-GUCCGCGUUCGg -3' miRNA: 3'- gGGCUGUu-CGGCccCAGGUGCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 124808 | 0.74 | 0.537461 |
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Target: 5'- gUCCGACGuccGCCGGGcGUCCACGaacagCGg -3' miRNA: 3'- -GGGCUGUu--CGGCCC-CAGGUGCaa---GCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 117343 | 0.74 | 0.52809 |
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Target: 5'- cCCCGGCuuGUCGGGGUCCGCcgUCa- -3' miRNA: 3'- -GGGCUGuuCGGCCCCAGGUGcaAGca -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 151671 | 0.79 | 0.328955 |
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Target: 5'- uCUCGACGgugaAGCUGGGGUCCACGggcugaaaUCGUg -3' miRNA: 3'- -GGGCUGU----UCGGCCCCAGGUGCa-------AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 112692 | 1.08 | 0.004276 |
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Target: 5'- cCCCGACAAGCCGGGGUCCACGUUCGUu -3' miRNA: 3'- -GGGCUGUUCGGCCCCAGGUGCAAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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