Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15805 | 3' | -57.7 | NC_004065.1 | + | 149065 | 0.67 | 0.890617 |
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Target: 5'- -gCGGCAGGCCgaucGGGGUCguCGUcacgaUCGUc -3' miRNA: 3'- ggGCUGUUCGG----CCCCAGguGCA-----AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 151671 | 0.79 | 0.328955 |
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Target: 5'- uCUCGACGgugaAGCUGGGGUCCACGggcugaaaUCGUg -3' miRNA: 3'- -GGGCUGU----UCGGCCCCAGGUGCa-------AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 152327 | 0.71 | 0.738443 |
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Target: 5'- -gCGACAGGaacaCGGGGcCCACGcUCGg -3' miRNA: 3'- ggGCUGUUCg---GCCCCaGGUGCaAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 159172 | 0.68 | 0.863505 |
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Target: 5'- gCUgGGCGAacaucCCGGGGUCCGCGUcuuucuucucuUCGa -3' miRNA: 3'- -GGgCUGUUc----GGCCCCAGGUGCA-----------AGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 163286 | 0.69 | 0.833404 |
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Target: 5'- uCgCGACGccGCCGGGGUCCgACGgcaCGg -3' miRNA: 3'- -GgGCUGUu-CGGCCCCAGG-UGCaa-GCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 182435 | 0.69 | 0.800681 |
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Target: 5'- gCUgGGC-AGCCgGGGGUCCugGUgaCGUa -3' miRNA: 3'- -GGgCUGuUCGG-CCCCAGGugCAa-GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 198450 | 0.72 | 0.652809 |
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Target: 5'- aCCGACGucGCCGc-GUCCGCGUUCGg -3' miRNA: 3'- gGGCUGUu-CGGCccCAGGUGCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 204759 | 0.69 | 0.817345 |
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Target: 5'- gCCCGACAGGCCGGGGaCaGCu----- -3' miRNA: 3'- -GGGCUGUUCGGCCCCaGgUGcaagca -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 218731 | 0.69 | 0.817345 |
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Target: 5'- gCCGACAucguAGCCGuacGUCCACG-UCGUg -3' miRNA: 3'- gGGCUGU----UCGGCcc-CAGGUGCaAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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