Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15805 | 3' | -57.7 | NC_004065.1 | + | 99970 | 0.66 | 0.934935 |
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Target: 5'- gCCgCGGCGugcucgGGCCGcGGGUCguCGUcuUCGUc -3' miRNA: 3'- -GG-GCUGU------UCGGC-CCCAGguGCA--AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 76306 | 0.67 | 0.914476 |
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Target: 5'- uCUCGuCGAGCCGGaGGaUCCGCcg-CGUg -3' miRNA: 3'- -GGGCuGUUCGGCC-CC-AGGUGcaaGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 59008 | 0.66 | 0.925135 |
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Target: 5'- -gCGAaAGGCCaGGGUCgGCgGUUCGUg -3' miRNA: 3'- ggGCUgUUCGGcCCCAGgUG-CAAGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 50909 | 0.69 | 0.817345 |
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Target: 5'- aCCGccacCAGGCaCGGGGUUCACGggCa- -3' miRNA: 3'- gGGCu---GUUCG-GCCCCAGGUGCaaGca -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 38086 | 0.7 | 0.79214 |
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Target: 5'- gCCCGugAGGCUGGagcuGUCgGCGUUCu- -3' miRNA: 3'- -GGGCugUUCGGCCc---CAGgUGCAAGca -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 31685 | 0.7 | 0.774678 |
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Target: 5'- cCCCGGCGAGCUGaGGGggaucuggccguUCCGCGgcaUGUg -3' miRNA: 3'- -GGGCUGUUCGGC-CCC------------AGGUGCaa-GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 26145 | 0.7 | 0.765772 |
|
Target: 5'- -aCGA-GAGCCGGGGUCUGCGUgccagcCGUc -3' miRNA: 3'- ggGCUgUUCGGCCCCAGGUGCAa-----GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 16439 | 0.67 | 0.908826 |
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Target: 5'- gCCCGagcccacuaGCGAGCCc-GGUaCCACGUUCGc -3' miRNA: 3'- -GGGC---------UGUUCGGccCCA-GGUGCAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 7314 | 0.67 | 0.896276 |
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Target: 5'- uCCUGACGaucgAGCCGGuGGUaCCGgacgcggcggagcCGUUCGg -3' miRNA: 3'- -GGGCUGU----UCGGCC-CCA-GGU-------------GCAAGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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