Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15805 | 3' | -57.7 | NC_004065.1 | + | 143084 | 0.68 | 0.863505 |
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Target: 5'- -gCGACGAGCgaaucuacgUGGGGUCCAUcUUCGc -3' miRNA: 3'- ggGCUGUUCG---------GCCCCAGGUGcAAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 159172 | 0.68 | 0.863505 |
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Target: 5'- gCUgGGCGAacaucCCGGGGUCCGCGUcuuucuucucuUCGa -3' miRNA: 3'- -GGgCUGUUc----GGCCCCAGGUGCA-----------AGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 130278 | 0.68 | 0.856247 |
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Target: 5'- cCCCGGCGAGUguuucgacaCGGGGaucuacagCCACGggCGc -3' miRNA: 3'- -GGGCUGUUCG---------GCCCCa-------GGUGCaaGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 204759 | 0.69 | 0.817345 |
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Target: 5'- gCCCGACAGGCCGGGGaCaGCu----- -3' miRNA: 3'- -GGGCUGUUCGGCCCCaGgUGcaagca -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 31685 | 0.7 | 0.774678 |
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Target: 5'- cCCCGGCGAGCUGaGGGggaucuggccguUCCGCGgcaUGUg -3' miRNA: 3'- -GGGCUGUUCGGC-CCC------------AGGUGCaa-GCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 152327 | 0.71 | 0.738443 |
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Target: 5'- -gCGACAGGaacaCGGGGcCCACGcUCGg -3' miRNA: 3'- ggGCUGUUCg---GCCCCaGGUGCaAGCa -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 151671 | 0.79 | 0.328955 |
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Target: 5'- uCUCGACGgugaAGCUGGGGUCCACGggcugaaaUCGUg -3' miRNA: 3'- -GGGCUGU----UCGGCCCCAGGUGCa-------AGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 112692 | 1.08 | 0.004276 |
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Target: 5'- cCCCGACAAGCCGGGGUCCACGUUCGUu -3' miRNA: 3'- -GGGCUGUUCGGCCCCAGGUGCAAGCA- -5' |
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| 15805 | 3' | -57.7 | NC_004065.1 | + | 16439 | 0.67 | 0.908826 |
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Target: 5'- gCCCGagcccacuaGCGAGCCc-GGUaCCACGUUCGc -3' miRNA: 3'- -GGGC---------UGUUCGGccCCA-GGUGCAAGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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