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miRNA: 3'- aACGACaACGCCG------CGCUCG------------UCGGCGg -5'
miRNA: 3'- aACGaCAA-------C-GCCGC-GCUCGUCGGCGG- -5'
miRNA: 3'- aaCGACa------ACGCCG--CGCU----CGUCGGCGG- -5'
miRNA: 3'- -AACGACAACGCCGCGCUCGUCGGCGG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 1581 | 3' | -60.4 | NC_001347.2 | + | 105938 | 0.73 | 0.367958 |
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Target: 5'- cUGCUGaUGCGGCuggaccGCGAGCgcagcgucggcgAGCCGCg -3' miRNA: 3'- aACGACaACGCCG------CGCUCG------------UCGGCGg -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 118276 | 0.74 | 0.344442 |
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Target: 5'- gUGCgGUUcgaagaaGaCGGCGaCGGGUAGCCGCCa -3' miRNA: 3'- aACGaCAA-------C-GCCGC-GCUCGUCGGCGG- -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 206992 | 0.74 | 0.323439 |
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Target: 5'- -cGCUGguaucgaUGCGGCagGCGAaugcGCAGCCGCUg -3' miRNA: 3'- aaCGACa------ACGCCG--CGCU----CGUCGGCGG- -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 185119 | 1.09 | 0.001377 |
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Target: 5'- gUUGCUGUUGCGGCGCGAGCAGCCGCCg -3' miRNA: 3'- -AACGACAACGCCGCGCUCGUCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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