miRNA: 3'- aaCGA------CAa----CGCCG-CGCU---CGUCGGCGG- -5'
miRNA: 3'- aaCGACaa--------CGCCGCGCu-CGUCGGCgg -5'
miRNA: 3'- aACG---ACaa--CGCCGCGCucGUCGGCGG- -5'
miRNA: 3'- aaCGACaaCGCCGcgcuCGuCGGCGG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1581 | 3' | -60.4 | NC_001347.2 | + | 67624 | 0.67 | 0.718776 |
|
Target: 5'- -cGCUucagacGUguccaGCGGCaGCGAcauGCGGCCGCUg -3' miRNA: 3'- aaCGA------CAa----CGCCG-CGCU---CGUCGGCGG- -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 157200 | 0.67 | 0.718776 |
|
Target: 5'- -aGCUGgugaucggccGCGGUGCGcuGCAGCCGg- -3' miRNA: 3'- aaCGACaa--------CGCCGCGCu-CGUCGGCgg -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 154814 | 0.67 | 0.728289 |
|
Target: 5'- gUGUcgaUGgccaGCGGCGCGcaCAGUCGCCg -3' miRNA: 3'- aACG---ACaa--CGCCGCGCucGUCGGCGG- -5' |
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| 1581 | 3' | -60.4 | NC_001347.2 | + | 222751 | 0.66 | 0.792269 |
|
Target: 5'- -cGCUGccGCGGCcauuGCcGCCGCCg -3' miRNA: 3'- aaCGACaaCGCCGcgcuCGuCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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