Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1581 | 5' | -54.7 | NC_001347.2 | + | 1921 | 0.68 | 0.921763 |
Target: 5'- uGGGCGcGGUGCUgccCGcGCGGUGGCUg- -3' miRNA: 3'- gCCCGU-UCACGAa--GU-CGUCGUCGAag -5' |
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1581 | 5' | -54.7 | NC_001347.2 | + | 66907 | 0.67 | 0.927208 |
Target: 5'- aGGaGCAgcuGGUGUUUCGauuGCGGCGGCa-- -3' miRNA: 3'- gCC-CGU---UCACGAAGU---CGUCGUCGaag -5' |
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1581 | 5' | -54.7 | NC_001347.2 | + | 173527 | 0.68 | 0.903989 |
Target: 5'- aGcGGCAAGUcGCguggCGGCGGCGGUg-- -3' miRNA: 3'- gC-CCGUUCA-CGaa--GUCGUCGUCGaag -5' |
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1581 | 5' | -54.7 | NC_001347.2 | + | 185153 | 1.1 | 0.003804 |
Target: 5'- aCGGGCAAGUGCUUCAGCAGCAGCUUCu -3' miRNA: 3'- -GCCCGUUCACGAAGUCGUCGUCGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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