miRNA: 3'- -CGUCCGGGACu--ACcUCUCGGUCgACU- -5'
miRNA: 3'- cgUCCGGGACUACCUcuCGGUCGacu -5'
miRNA: 3'- cGUCCGGGACUa---------CCUCUCGGUCgaCU- -5'
miRNA: 3'- -CGUcCGGGACUaCCuCUCGGuCGACU- -5'
miRNA: 3'- -CGUCCGGGACUACCUCUCGGUCGACU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15817 | 5' | -58.4 | NC_004065.1 | + | 195797 | 0.7 | 0.649627 |
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Target: 5'- cGCGGGCCCUGcgcUGaAGGGUCGGgUGGg -3' miRNA: 3'- -CGUCCGGGACu--ACcUCUCGGUCgACU- -5' |
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| 15817 | 5' | -58.4 | NC_004065.1 | + | 123414 | 0.71 | 0.590186 |
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Target: 5'- -aGGGCCCUGAUGGA--GUCGGCg-- -3' miRNA: 3'- cgUCCGGGACUACCUcuCGGUCGacu -5' |
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| 15817 | 5' | -58.4 | NC_004065.1 | + | 48225 | 0.73 | 0.49397 |
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Target: 5'- cUAGGCCCUGAcagaaaaaagGGAGAGUUAGgaGAg -3' miRNA: 3'- cGUCCGGGACUa---------CCUCUCGGUCgaCU- -5' |
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| 15817 | 5' | -58.4 | NC_004065.1 | + | 71993 | 0.76 | 0.34241 |
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Target: 5'- cGCAuGCCCUGGaGGuGAGCCuGCUGGa -3' miRNA: 3'- -CGUcCGGGACUaCCuCUCGGuCGACU- -5' |
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| 15817 | 5' | -58.4 | NC_004065.1 | + | 106849 | 1.11 | 0.001754 |
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Target: 5'- cGCAGGCCCUGAUGGAGAGCCAGCUGAa -3' miRNA: 3'- -CGUCCGGGACUACCUCUCGGUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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