Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15817 | 5' | -58.4 | NC_004065.1 | + | 151089 | 0.66 | 0.865152 |
Target: 5'- cGCGGG-CCUGGUGGcgcccGAGCuCGGCg-- -3' miRNA: 3'- -CGUCCgGGACUACCu----CUCG-GUCGacu -5' |
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15817 | 5' | -58.4 | NC_004065.1 | + | 164194 | 0.7 | 0.65953 |
Target: 5'- cGCGGGCCCUcAUGGAucucgccuuuacGAGCC-GCaUGAc -3' miRNA: 3'- -CGUCCGGGAcUACCU------------CUCGGuCG-ACU- -5' |
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15817 | 5' | -58.4 | NC_004065.1 | + | 192479 | 0.66 | 0.857767 |
Target: 5'- -gGGuGCCCcgacgaUGAUGGAGGGCUGuugcacGCUGAg -3' miRNA: 3'- cgUC-CGGG------ACUACCUCUCGGU------CGACU- -5' |
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15817 | 5' | -58.4 | NC_004065.1 | + | 195797 | 0.7 | 0.649627 |
Target: 5'- cGCGGGCCCUGcgcUGaAGGGUCGGgUGGg -3' miRNA: 3'- -CGUCCGGGACu--ACcUCUCGGUCgACU- -5' |
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15817 | 5' | -58.4 | NC_004065.1 | + | 209552 | 0.68 | 0.756103 |
Target: 5'- cGCGGGa-CUGAaGGAGgcccGGCCGGCUGc -3' miRNA: 3'- -CGUCCggGACUaCCUC----UCGGUCGACu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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