Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15821 | 3' | -57.9 | NC_004065.1 | + | 210738 | 0.66 | 0.849772 |
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Target: 5'- ----cGGACGGuccaGAUCCCCCGucGCGCGa -3' miRNA: 3'- uuguuCCUGUC----CUAGGGGGU--CGCGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 28880 | 0.66 | 0.857484 |
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Target: 5'- uGACGAGGACugcuGGcgCCCCgAGUcgGCGg -3' miRNA: 3'- -UUGUUCCUGu---CCuaGGGGgUCG--CGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 66604 | 0.66 | 0.857484 |
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Target: 5'- gGACGcGGACAGGAUCCggagccgcgggcUgCGGCGCu- -3' miRNA: 3'- -UUGUuCCUGUCCUAGG------------GgGUCGCGua -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 67207 | 0.66 | 0.872306 |
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Target: 5'- --uGGGGACGGaGAUCCCCaCGGUGa-- -3' miRNA: 3'- uugUUCCUGUC-CUAGGGG-GUCGCgua -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 29109 | 0.66 | 0.872306 |
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Target: 5'- uGACGugGGGACacuuGGGAggccggUCgCCCAGCGCGa -3' miRNA: 3'- -UUGU--UCCUG----UCCU------AGgGGGUCGCGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 117169 | 0.66 | 0.879406 |
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Target: 5'- cAGCGccGACGGGGUCCgCCCcuGGgGCAg -3' miRNA: 3'- -UUGUucCUGUCCUAGG-GGG--UCgCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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