Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
15821 | 3' | -57.9 | NC_004065.1 | + | 126802 | 0.7 | 0.635402 |
Target: 5'- cGCGAGGugGGGAUCccggCCCCGGUGg-- -3' miRNA: 3'- uUGUUCCugUCCUAG----GGGGUCGCgua -5' |
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15821 | 3' | -57.9 | NC_004065.1 | + | 120569 | 0.71 | 0.595117 |
Target: 5'- cGCGAgcGGGCGGGA-CCUCCGGCGCc- -3' miRNA: 3'- uUGUU--CCUGUCCUaGGGGGUCGCGua -5' |
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15821 | 3' | -57.9 | NC_004065.1 | + | 121617 | 0.72 | 0.564161 |
Target: 5'- cGguAGGGCAGGAUgCCCCCgaggauggcguugAGCGCGUc -3' miRNA: 3'- uUguUCCUGUCCUA-GGGGG-------------UCGCGUA- -5' |
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15821 | 3' | -57.9 | NC_004065.1 | + | 209122 | 0.72 | 0.545395 |
Target: 5'- cAACGAGGcgaaaGCGagcGGAUUCCCCAGCGCc- -3' miRNA: 3'- -UUGUUCC-----UGU---CCUAGGGGGUCGCGua -5' |
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15821 | 3' | -57.9 | NC_004065.1 | + | 513 | 0.73 | 0.487649 |
Target: 5'- aGGCGGGGugAGGcgCCCCgcCAGCGUGUg -3' miRNA: 3'- -UUGUUCCugUCCuaGGGG--GUCGCGUA- -5' |
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15821 | 3' | -57.9 | NC_004065.1 | + | 104210 | 1.03 | 0.005968 |
Target: 5'- cAACAAGGACAGGAUCCCCCAGCGCAUc -3' miRNA: 3'- -UUGUUCCUGUCCUAGGGGGUCGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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