Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15821 | 3' | -57.9 | NC_004065.1 | + | 202240 | 0.68 | 0.753909 |
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Target: 5'- uGCuGGGGCAGGAggCCCUCcagacggcGGCGCAg -3' miRNA: 3'- uUGuUCCUGUCCUa-GGGGG--------UCGCGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 209122 | 0.72 | 0.545395 |
|
Target: 5'- cAACGAGGcgaaaGCGagcGGAUUCCCCAGCGCc- -3' miRNA: 3'- -UUGUUCC-----UGU---CCUAGGGGGUCGCGua -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 210738 | 0.66 | 0.849772 |
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Target: 5'- ----cGGACGGuccaGAUCCCCCGucGCGCGa -3' miRNA: 3'- uuguuCCUGUC----CUAGGGGGU--CGCGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 211837 | 0.68 | 0.752962 |
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Target: 5'- gGACAAGGcuaucgcACGGGAgUCCCggCCGGCGCu- -3' miRNA: 3'- -UUGUUCC-------UGUCCU-AGGG--GGUCGCGua -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 224666 | 0.67 | 0.84187 |
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Target: 5'- --aGGGGAUAGGGaccgUgCCaCCAGCGCAUc -3' miRNA: 3'- uugUUCCUGUCCU----AgGG-GGUCGCGUA- -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 229590 | 0.68 | 0.772609 |
|
Target: 5'- cGCGAGGGCggcuccagGGGGUucaCCCCCcgGGCGCGg -3' miRNA: 3'- uUGUUCCUG--------UCCUA---GGGGG--UCGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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