Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15821 | 3' | -57.9 | NC_004065.1 | + | 202240 | 0.68 | 0.753909 |
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Target: 5'- uGCuGGGGCAGGAggCCCUCcagacggcGGCGCAg -3' miRNA: 3'- uUGuUCCUGUCCUa-GGGGG--------UCGCGUa -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 160679 | 0.69 | 0.733826 |
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Target: 5'- -cCGAGGGCAGGuugCCCUccgccagcugcuuCAGCGCGUu -3' miRNA: 3'- uuGUUCCUGUCCua-GGGG-------------GUCGCGUA- -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 121617 | 0.72 | 0.564161 |
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Target: 5'- cGguAGGGCAGGAUgCCCCCgaggauggcguugAGCGCGUc -3' miRNA: 3'- uUguUCCUGUCCUA-GGGGG-------------UCGCGUA- -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 209122 | 0.72 | 0.545395 |
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Target: 5'- cAACGAGGcgaaaGCGagcGGAUUCCCCAGCGCc- -3' miRNA: 3'- -UUGUUCC-----UGU---CCUAGGGGGUCGCGua -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 104210 | 1.03 | 0.005968 |
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Target: 5'- cAACAAGGACAGGAUCCCCCAGCGCAUc -3' miRNA: 3'- -UUGUUCCUGUCCUAGGGGGUCGCGUA- -5' |
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| 15821 | 3' | -57.9 | NC_004065.1 | + | 28880 | 0.66 | 0.857484 |
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Target: 5'- uGACGAGGACugcuGGcgCCCCgAGUcgGCGg -3' miRNA: 3'- -UUGUUCCUGu---CCuaGGGGgUCG--CGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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