Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15828 | 5' | -60.2 | NC_004065.1 | + | 169792 | 0.68 | 0.66986 |
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Target: 5'- gAGCGGGAacgggaacggGCU-CUGGGAGCUuGACGGg -3' miRNA: 3'- -UCGCUCU----------CGAgGGCCCUCGA-CUGCUu -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 49584 | 0.68 | 0.679603 |
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Target: 5'- cGCGAcGAGaaagCCCGGGuccCUGACGAAc -3' miRNA: 3'- uCGCU-CUCga--GGGCCCuc-GACUGCUU- -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 185831 | 0.68 | 0.679603 |
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Target: 5'- uAGUGAGAGuCUCucggguCCGGGGGCgUGAgGGAc -3' miRNA: 3'- -UCGCUCUC-GAG------GGCCCUCG-ACUgCUU- -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 41304 | 0.68 | 0.68931 |
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Target: 5'- -aCG-GAGCUCuuGGaGAGCcUGACGAAc -3' miRNA: 3'- ucGCuCUCGAGggCC-CUCG-ACUGCUU- -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 91758 | 0.67 | 0.718138 |
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Target: 5'- cGGCGGGAGCga-CGGGGGCgaGGCuGGAg -3' miRNA: 3'- -UCGCUCUCGaggGCCCUCGa-CUG-CUU- -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 224458 | 0.67 | 0.746357 |
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Target: 5'- gGGcCGGGcAGCUCaaCCGGGAGCUGGu--- -3' miRNA: 3'- -UC-GCUC-UCGAG--GGCCCUCGACUgcuu -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 139575 | 0.66 | 0.79144 |
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Target: 5'- cGCGcAGAGCaCUCGGGAGgUGGCc-- -3' miRNA: 3'- uCGC-UCUCGaGGGCCCUCgACUGcuu -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 17358 | 0.66 | 0.79144 |
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Target: 5'- cGCGAGAGaCUgUUGuuGGGGUUGACGAGc -3' miRNA: 3'- uCGCUCUC-GAgGGC--CCUCGACUGCUU- -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 136604 | 0.66 | 0.808605 |
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Target: 5'- uGGCGuuGGCUCCUGGGuGCagaucaagGAUGGu -3' miRNA: 3'- -UCGCucUCGAGGGCCCuCGa-------CUGCUu -5' |
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| 15828 | 5' | -60.2 | NC_004065.1 | + | 80066 | 0.73 | 0.384173 |
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Target: 5'- uAGCagGAGAGCUCuuGGGAGagGACGGAg -3' miRNA: 3'- -UCG--CUCUCGAGggCCCUCgaCUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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