Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15829 | 5' | -62.7 | NC_004065.1 | + | 77118 | 0.77 | 0.213571 |
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Target: 5'- aGCGCCGCCGAcaucGUCAGacggggcugcgguuGGGGUUGGg -3' miRNA: 3'- gCGCGGCGGCUa---CAGUC--------------CCCCAGCCa -5' |
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| 15829 | 5' | -62.7 | NC_004065.1 | + | 67654 | 0.7 | 0.510135 |
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Target: 5'- aCGCGUCGgUGAUGUCGGGGagcGG-CGGc -3' miRNA: 3'- -GCGCGGCgGCUACAGUCCC---CCaGCCa -5' |
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| 15829 | 5' | -62.7 | NC_004065.1 | + | 33282 | 0.68 | 0.593282 |
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Target: 5'- gCGCaGCaCGCCGAUGUUGGGGuaGG-CGGc -3' miRNA: 3'- -GCG-CG-GCGGCUACAGUCCC--CCaGCCa -5' |
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| 15829 | 5' | -62.7 | NC_004065.1 | + | 32882 | 0.73 | 0.368436 |
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Target: 5'- uCGCGCUccucguccgagauGCCGuccUGUC-GGGGGUCGGa -3' miRNA: 3'- -GCGCGG-------------CGGCu--ACAGuCCCCCAGCCa -5' |
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| 15829 | 5' | -62.7 | NC_004065.1 | + | 30093 | 0.66 | 0.706291 |
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Target: 5'- aGCGCgCGCUGAugUGUCAGGGa--CGGa -3' miRNA: 3'- gCGCG-GCGGCU--ACAGUCCCccaGCCa -5' |
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| 15829 | 5' | -62.7 | NC_004065.1 | + | 2742 | 0.7 | 0.528257 |
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Target: 5'- uGUuCCgaGCgGAcGUCGGGGGGUCGGUc -3' miRNA: 3'- gCGcGG--CGgCUaCAGUCCCCCAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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