Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1583 | 5' | -58.3 | NC_001347.2 | + | 228555 | 0.66 | 0.876309 |
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Target: 5'- cGUGUGCGCGaaagccuGUgCCGCGGCAgCGUa -3' miRNA: 3'- aCGCACGCGUc------CAgGGCGUUGU-GCAg -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 126393 | 0.66 | 0.876309 |
|
Target: 5'- -aCGUGCGCAGcUUCUGCAcGCGCGa- -3' miRNA: 3'- acGCACGCGUCcAGGGCGU-UGUGCag -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 184233 | 0.66 | 0.876309 |
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Target: 5'- aGCGUGuCGCAGcGUUcgCCGCcguuGC-CGUCg -3' miRNA: 3'- aCGCAC-GCGUC-CAG--GGCGu---UGuGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 90224 | 0.66 | 0.876309 |
|
Target: 5'- gGCGUG-GCGGcGUCguuuuUCGgAGCGCGUCg -3' miRNA: 3'- aCGCACgCGUC-CAG-----GGCgUUGUGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 38277 | 0.66 | 0.86928 |
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Target: 5'- cGCGUGCGC--GUCCgCGUuaGGCACG-Ca -3' miRNA: 3'- aCGCACGCGucCAGG-GCG--UUGUGCaG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 187349 | 0.66 | 0.86928 |
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Target: 5'- gUGCGuUGCGUGG---CCGUGGCGCGUCg -3' miRNA: 3'- -ACGC-ACGCGUCcagGGCGUUGUGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 40985 | 0.66 | 0.896166 |
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Target: 5'- cUGCuUGCGCacAGG-CCCGcCAACACcUCu -3' miRNA: 3'- -ACGcACGCG--UCCaGGGC-GUUGUGcAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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