Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1583 | 5' | -58.3 | NC_001347.2 | + | 1239 | 0.66 | 0.883136 |
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Target: 5'- cGCGccaUGCGCAaguGGUCgcagCGCGACGCGg- -3' miRNA: 3'- aCGC---ACGCGU---CCAGg---GCGUUGUGCag -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 142207 | 0.66 | 0.883136 |
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Target: 5'- aGCGUGCGCgAGGagaUCCC----CGCGUCc -3' miRNA: 3'- aCGCACGCG-UCC---AGGGcguuGUGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 184425 | 0.66 | 0.883136 |
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Target: 5'- aGCG-GCGcCGGGUCgCUGUGACGgagcuCGUCg -3' miRNA: 3'- aCGCaCGC-GUCCAG-GGCGUUGU-----GCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 136464 | 0.66 | 0.894901 |
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Target: 5'- gGCGcaUGCGCAGGgcgacccUCCCGCGggcccuggcccgcGCAUG-Cg -3' miRNA: 3'- aCGC--ACGCGUCC-------AGGGCGU-------------UGUGCaG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 40985 | 0.66 | 0.896166 |
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Target: 5'- cUGCuUGCGCacAGG-CCCGcCAACACcUCu -3' miRNA: 3'- -ACGcACGCG--UCCaGGGC-GUUGUGcAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 87660 | 0.66 | 0.896166 |
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Target: 5'- cGCGUGCGgauCAGG-CCCGaCAuccagaccgugaGCACcGUCu -3' miRNA: 3'- aCGCACGC---GUCCaGGGC-GU------------UGUG-CAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 22506 | 0.66 | 0.901752 |
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Target: 5'- cGUGUGCaCGGGcUUuuGCGgagaaagGCGCGUCa -3' miRNA: 3'- aCGCACGcGUCC-AGggCGU-------UGUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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