Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1583 | 5' | -58.3 | NC_001347.2 | + | 190780 | 0.67 | 0.831278 |
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Target: 5'- uUGCGgcugaauCGCAGGUcguaCCCGCGcuGCGCGcUCg -3' miRNA: 3'- -ACGCac-----GCGUCCA----GGGCGU--UGUGC-AG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 185480 | 0.67 | 0.831278 |
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Target: 5'- gGCGUuguggcGCGCAGGggcUCCGaCGugGCGUUa -3' miRNA: 3'- aCGCA------CGCGUCCa--GGGC-GUugUGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 198084 | 0.67 | 0.83924 |
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Target: 5'- cGCGgcgGuCGCGacggugacGGUCCCGCucguCGCGUUg -3' miRNA: 3'- aCGCa--C-GCGU--------CCAGGGCGuu--GUGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 157272 | 0.67 | 0.847027 |
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Target: 5'- uUGCG-GCGCAgacgucggcGGUCCUccagcaGCAGCugGUg -3' miRNA: 3'- -ACGCaCGCGU---------CCAGGG------CGUUGugCAg -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 163633 | 0.67 | 0.862053 |
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Target: 5'- cGCGgcUGCcCGGGUCgCGCAccaGCGUCu -3' miRNA: 3'- aCGC--ACGcGUCCAGgGCGUug-UGCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 151721 | 0.67 | 0.862053 |
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Target: 5'- cGUGUGCGUgucgAGGaCCUGguGCuuuCGUCg -3' miRNA: 3'- aCGCACGCG----UCCaGGGCguUGu--GCAG- -5' |
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| 1583 | 5' | -58.3 | NC_001347.2 | + | 40985 | 0.66 | 0.896166 |
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Target: 5'- cUGCuUGCGCacAGG-CCCGcCAACACcUCu -3' miRNA: 3'- -ACGcACGCG--UCCaGGGC-GUUGUGcAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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