Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15848 | 5' | -56.3 | NC_004065.1 | + | 196648 | 0.66 | 0.934927 |
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Target: 5'- aUCCAGGGGCcaguAGCgCgCGGcGUCuCUCACg -3' miRNA: 3'- -AGGUCCUCG----UCG-G-GCUaUAGcGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 189085 | 0.66 | 0.939659 |
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Target: 5'- -gCGGGGGCucgaggaccucGCCCGGUGUCucuguGCUCAg -3' miRNA: 3'- agGUCCUCGu----------CGGGCUAUAG-----CGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 181356 | 0.66 | 0.944164 |
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Target: 5'- uUCCAGGA-CGGCCUGc-GUCgGCUCGa -3' miRNA: 3'- -AGGUCCUcGUCGGGCuaUAG-CGAGUg -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 43096 | 0.66 | 0.944164 |
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Target: 5'- gUCCGGGcauccGCgacccGGCgCCGAUccgCGCUCGCg -3' miRNA: 3'- -AGGUCCu----CG-----UCG-GGCUAua-GCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 169126 | 0.66 | 0.944164 |
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Target: 5'- gCCGcGGccAGCAGuuCCCGAga-CGCUCGCg -3' miRNA: 3'- aGGU-CC--UCGUC--GGGCUauaGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 61980 | 0.66 | 0.946759 |
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Target: 5'- aCCAGGAGCAGCaCCagcagccccCGCgaaCACa -3' miRNA: 3'- aGGUCCUCGUCG-GGcuaua----GCGa--GUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 145739 | 0.66 | 0.948444 |
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Target: 5'- -gCGGGAGCGGUgaugagugcgCCGAUcgcGUCGCgCGCc -3' miRNA: 3'- agGUCCUCGUCG----------GGCUA---UAGCGaGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 150178 | 0.66 | 0.948444 |
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Target: 5'- cUUguGGAugcGCAGCUCGuuGUGUCGUUUGCg -3' miRNA: 3'- -AGguCCU---CGUCGGGC--UAUAGCGAGUG- -5' |
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| 15848 | 5' | -56.3 | NC_004065.1 | + | 170947 | 0.66 | 0.948444 |
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Target: 5'- gCC-GGcGC-GCCgCGAUcUCGCUCACa -3' miRNA: 3'- aGGuCCuCGuCGG-GCUAuAGCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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